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R&D Systems gas6 axl signal modulators

(A) Cytokine array analysis of the K. pneumoniae infection model based on the Transwell insert co-culture system consisting of Caco-2 cells and RAW264.7 macrophages. Quantification of Axl signals using a laser scanner. Data are presented as the mean ± SD. ** p < 0.01. (B and C) Secretion of Axl (B) and <t>Gas6</t> (C) by Caco-2 cells or RAW264.7 macrophages. Culture supernatants or lysates of K. pneumoniae were added to Caco-2 cells or RAW264.7 macrophages for 12 h. The culture media were then collected for ELISA analysis of Axl or Gas6 levels. Data are presented as the mean ± SD. ** p < 0.01. (D) K. pneumoniae -infected Caco-2 cells grown on a Transwell insert in the presence or absence of RAW264.7 macrophages were immunostained with anti-Gas6 and anti-Axl antibodies. Scale bar = 50 μm.

Gas6 Axl Signal Modulators, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gas6+axl+signal+modulators/Mouse+Gas6+Antibody/bio_rxiv__2023__01__19__524842-128-45-24
Average 94 stars, based on 1 article reviews

gas6 axl signal modulators - by Bioz Stars, 2026-10

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1) Product Images from "Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae , to the liver"

Article Title: Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae , to the liver

Journal: bioRxiv

doi: 10.1101/2023.01.19.524842

Figure Legend Snippet: (A) Cytokine array analysis of the K. pneumoniae infection model based on the Transwell insert co-culture system consisting of Caco-2 cells and RAW264.7 macrophages. Quantification of Axl signals using a laser scanner. Data are presented as the mean ± SD. ** p < 0.01. (B and C) Secretion of Axl (B) and Gas6 (C) by Caco-2 cells or RAW264.7 macrophages. Culture supernatants or lysates of K. pneumoniae were added to Caco-2 cells or RAW264.7 macrophages for 12 h. The culture media were then collected for ELISA analysis of Axl or Gas6 levels. Data are presented as the mean ± SD. ** p < 0.01. (D) K. pneumoniae -infected Caco-2 cells grown on a Transwell insert in the presence or absence of RAW264.7 macrophages were immunostained with anti-Gas6 and anti-Axl antibodies. Scale bar = 50 μm.

Techniques Used: Infection, Co-Culture Assay, Enzyme-linked Immunosorbent Assay

(A) Western blotting was performed to detect the expression of Axl, Gas6, ZO-1, and occludin in Caco-2 cells infected with K. pneumoniae in the presence of an Axl inhibitor (R428) or an anti-Gas6 antibody. Prior to K. pneumoniae infection, cells were treated for 3 h with Axl inhibitor (R428; 20 nM) or 1 μg of anti-Gas6 antibody. (B and C) Axl inhibitor (R428; 20 nM) or 1 μg of anti-Gas6 antibody were added to Caco-2 cells grown on the insert in in the presence or absence of RAW264.7 macrophages for 3 h prior to K. pneumoniae infection. Next, Caco-2 cells were immunostained with an anti-ZO-1 antibody (B), an anti-occludin antibody (B), and an anti- Klebsiella pneumoniae antibody (C). Scale bar = 50 μm. Immunostaining intensities of ZO-1 (B), occludin (B), and K. pneumoniae (C) were analyzed by ImageJ software. Data are presented as the mean ± SD. ** p < 0.01.

Figure Legend Snippet: (A) Western blotting was performed to detect the expression of Axl, Gas6, ZO-1, and occludin in Caco-2 cells infected with K. pneumoniae in the presence of an Axl inhibitor (R428) or an anti-Gas6 antibody. Prior to K. pneumoniae infection, cells were treated for 3 h with Axl inhibitor (R428; 20 nM) or 1 μg of anti-Gas6 antibody. (B and C) Axl inhibitor (R428; 20 nM) or 1 μg of anti-Gas6 antibody were added to Caco-2 cells grown on the insert in in the presence or absence of RAW264.7 macrophages for 3 h prior to K. pneumoniae infection. Next, Caco-2 cells were immunostained with an anti-ZO-1 antibody (B), an anti-occludin antibody (B), and an anti- Klebsiella pneumoniae antibody (C). Scale bar = 50 μm. Immunostaining intensities of ZO-1 (B), occludin (B), and K. pneumoniae (C) were analyzed by ImageJ software. Data are presented as the mean ± SD. ** p < 0.01.

Techniques Used: Western Blot, Expressing, Infection, Immunostaining, Software

(A and B) Sections of cecal mucosa (A) or liver (B) were from mice aged 15 or 57 weeks at 2 days after infection with K. pneumoniae ATCC43816 pmCherry and immunostained with an anti-Gas6 antibody and an anti-Axl antibody. Scale bar = 50 μm. (C) Detection of Axl, Gas6, ZO-1, and occludin by western blotting. Cecum and liver tissues were collected, homogenized, and analyzed using an anti-Axl antibody, an anti-Gas6 antibody, an anti-ZO-1 antibody, or an anti-occludin antibody. Signal intensity was analyzed by ImageJ software. Data are presented as the mean ± SD (n = 5 per group). NS: not significant, * p < 0.05, ** p < 0.01. (D) Linear correlation between Gas6 expression and Axl expression in the cecum or liver of mice aged 15 (red circles) or 57 (blue circles) weeks infected with K. pneumoniae ATCC43816 pmCherry. r > 0.70 and p < 0.05. (E) The population of CD11b+F4/80+ macrophages in the intestinal mucosa of young (14-week-old) and old (56-week-old) mice was examined by staining with anti-F4/80 and anti-CD11b antibodies. The percentage of F4/80-positive/CX3CR1-negative cells in 14-week-old and 56-week-old mice was 29.5% and 8.87%, respectively. Data are representative of four mice.

Figure Legend Snippet: (A and B) Sections of cecal mucosa (A) or liver (B) were from mice aged 15 or 57 weeks at 2 days after infection with K. pneumoniae ATCC43816 pmCherry and immunostained with an anti-Gas6 antibody and an anti-Axl antibody. Scale bar = 50 μm. (C) Detection of Axl, Gas6, ZO-1, and occludin by western blotting. Cecum and liver tissues were collected, homogenized, and analyzed using an anti-Axl antibody, an anti-Gas6 antibody, an anti-ZO-1 antibody, or an anti-occludin antibody. Signal intensity was analyzed by ImageJ software. Data are presented as the mean ± SD (n = 5 per group). NS: not significant, * p < 0.05, ** p < 0.01. (D) Linear correlation between Gas6 expression and Axl expression in the cecum or liver of mice aged 15 (red circles) or 57 (blue circles) weeks infected with K. pneumoniae ATCC43816 pmCherry. r > 0.70 and p < 0.05. (E) The population of CD11b+F4/80+ macrophages in the intestinal mucosa of young (14-week-old) and old (56-week-old) mice was examined by staining with anti-F4/80 and anti-CD11b antibodies. The percentage of F4/80-positive/CX3CR1-negative cells in 14-week-old and 56-week-old mice was 29.5% and 8.87%, respectively. Data are representative of four mice.

Techniques Used: Infection, Western Blot, Software, Expressing, Staining

(A) Administration of Gas6 recombinant protein to Caco-2 cells to analyze the expression of Axl, ZO-1, and occludin. Addition of Gas6 recombinant protein to the apical surface (left scheme) or the basolateral side (right scheme) of Caco-2 cells. (B) Western blot analysis to detect Axl, Gas6, ZO-1, and occludin in Caco-2 cells treated with human Gas6 recombinant protein from the apical or basolateral sides. (C) Western blot analysis was performed to detect Axl, Gas6, ZO-1, and occludin. Gas6 recombinant protein (1 μg) was added to the apical side of Caco-2 cells grown in a Transwell co-culture system for 3 h prior to K. pneumoniae infection. (D and E) Gas6 recombinant protein (1 μg) was added to Caco-2 cells grown on the Transwell insert for 3 h prior to K. pneumoniae infection. Next, Caco-2 cells were immunostained with an anti-ZO-1 antibody (C), an anti-occludin antibody (C), an anti- Klebsiella pneumoniae antibody (D), and an anti-E-cadherin antibody. The signal intensities of ZO-1, occluding, E-cadherin, and K. pneumoniae were analyzed by ImageJ software. Data are presented as the mean ± SD. NS: not significant, ** p < 0.01.

Figure Legend Snippet: (A) Administration of Gas6 recombinant protein to Caco-2 cells to analyze the expression of Axl, ZO-1, and occludin. Addition of Gas6 recombinant protein to the apical surface (left scheme) or the basolateral side (right scheme) of Caco-2 cells. (B) Western blot analysis to detect Axl, Gas6, ZO-1, and occludin in Caco-2 cells treated with human Gas6 recombinant protein from the apical or basolateral sides. (C) Western blot analysis was performed to detect Axl, Gas6, ZO-1, and occludin. Gas6 recombinant protein (1 μg) was added to the apical side of Caco-2 cells grown in a Transwell co-culture system for 3 h prior to K. pneumoniae infection. (D and E) Gas6 recombinant protein (1 μg) was added to Caco-2 cells grown on the Transwell insert for 3 h prior to K. pneumoniae infection. Next, Caco-2 cells were immunostained with an anti-ZO-1 antibody (C), an anti-occludin antibody (C), an anti- Klebsiella pneumoniae antibody (D), and an anti-E-cadherin antibody. The signal intensities of ZO-1, occluding, E-cadherin, and K. pneumoniae were analyzed by ImageJ software. Data are presented as the mean ± SD. NS: not significant, ** p < 0.01.

Techniques Used: Recombinant, Expressing, Western Blot, Co-Culture Assay, Infection, Software

(A) Treatment scheme used to analyze the effect of Gas6 recombinant protein on the susceptibility of 57-week-old mice to infection by K. pneumoniae . Antibiotics were administered 4 weeks before administration of Gas6 recombinant protein. Gas6 recombinant protein (125 μg protein/kg) was administered intraperitoneally to 57-week-old mice three times every 24 h prior to bacterial infection. (B) Effect of Gas6 recombinant protein on survival of 57-week-old mice infected with K. pneumoniae ATCC43816 pmCherry. Each mouse was orally inoculated with K. pneumoniae ATCC43816 pmCherry (5 × 10 7 bacteria). p -values were determined using the log-rank test (n = 6 per group). (C) Bacterial counts in cecum and liver tissues were determined 2 days post-infection. Cecum and liver tissues were homogenized in PBS. The homogenates were plated on LB agar containing 400 μg/mL ampicillin and the number of CFU was counted. Data are presented as the mean ± SD. (n = 6 per group). * p < 0.05, ** p < 0.01. (D) Detection of Axl, Gas6, ZO-1, and occludin by western blotting. Cecum tissues were collected, homogenized, and analyzed by western blotting with anti-Axl, anti-Gas6, anti-ZO-1, or anti-occludin antibodies. Signal intensity was analyzed by ImageJ software. Data are presented as the mean ± SD (n = 5 per group). NS: not significant, * p < 0.05, ** p < 0.01.

Figure Legend Snippet: (A) Treatment scheme used to analyze the effect of Gas6 recombinant protein on the susceptibility of 57-week-old mice to infection by K. pneumoniae . Antibiotics were administered 4 weeks before administration of Gas6 recombinant protein. Gas6 recombinant protein (125 μg protein/kg) was administered intraperitoneally to 57-week-old mice three times every 24 h prior to bacterial infection. (B) Effect of Gas6 recombinant protein on survival of 57-week-old mice infected with K. pneumoniae ATCC43816 pmCherry. Each mouse was orally inoculated with K. pneumoniae ATCC43816 pmCherry (5 × 10 7 bacteria). p -values were determined using the log-rank test (n = 6 per group). (C) Bacterial counts in cecum and liver tissues were determined 2 days post-infection. Cecum and liver tissues were homogenized in PBS. The homogenates were plated on LB agar containing 400 μg/mL ampicillin and the number of CFU was counted. Data are presented as the mean ± SD. (n = 6 per group). * p < 0.05, ** p < 0.01. (D) Detection of Axl, Gas6, ZO-1, and occludin by western blotting. Cecum tissues were collected, homogenized, and analyzed by western blotting with anti-Axl, anti-Gas6, anti-ZO-1, or anti-occludin antibodies. Signal intensity was analyzed by ImageJ software. Data are presented as the mean ± SD (n = 5 per group). NS: not significant, * p < 0.05, ** p < 0.01.

Techniques Used: Recombinant, Infection, Western Blot, Software

Recombinant:

Article Title: Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae , to the liver
Article Snippet: All animal experiments were approved by the Keio University (Tokyo, Japan) Animal Research Committee (no. 19048) and the Tokai University (Kanagawa, Japan) Animal Research Committee (no. 222002) and were conducted in accordance with the “Act on Welfare and Management of Animals of Japan,” “Standards relating to the Care and Keeping and Reducing Pain of Laboratory Animals,” “Standards relating to the Methods of Destruction of Animals,” “Guidelines for Proper Conduct of Animal Experiments,” and “Fundamental Guidelines for Proper Conduct of Animal Experiments.” .. An Axl inhibitor (R428) (Abcam, Cambridge, UK, cat# ab141364), recombinant human Gas6 protein (R&D Systems, Minneapolis, MN, USA, cat# 885-GSB), recombinant mouse Gas6 protein (R&D Systems, cat# 986-GS) (used in administration experiments in mice), and an anti-Gas6 antibody (R&D Systems, cat# AF986) were used as Gas6/Axl signal modulators. .. Rhodamine phalloidin (Invitrogen, Waltham, MA, USA, R415) was used to stain the polymerized actin.

Image Search Results

Journal: bioRxiv

Article Title: Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae , to the liver

doi: 10.1101/2023.01.19.524842

Figure Lengend Snippet: (A) Cytokine array analysis of the K. pneumoniae infection model based on the Transwell insert co-culture system consisting of Caco-2 cells and RAW264.7 macrophages. Quantification of Axl signals using a laser scanner. Data are presented as the mean ± SD. ** p < 0.01. (B and C) Secretion of Axl (B) and Gas6 (C) by Caco-2 cells or RAW264.7 macrophages. Culture supernatants or lysates of K. pneumoniae were added to Caco-2 cells or RAW264.7 macrophages for 12 h. The culture media were then collected for ELISA analysis of Axl or Gas6 levels. Data are presented as the mean ± SD. ** p < 0.01. (D) K. pneumoniae -infected Caco-2 cells grown on a Transwell insert in the presence or absence of RAW264.7 macrophages were immunostained with anti-Gas6 and anti-Axl antibodies. Scale bar = 50 μm.

Article Snippet: An Axl inhibitor (R428) (Abcam, Cambridge, UK, cat# ab141364), recombinant human Gas6 protein (R&D Systems, Minneapolis, MN, USA, cat# 885-GSB), recombinant mouse Gas6 protein (R&D Systems, cat# 986-GS) (used in administration experiments in mice), and an anti-Gas6 antibody (R&D Systems, cat# AF986) were used as Gas6/Axl signal modulators.

Techniques: Infection, Co-Culture Assay, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae , to the liver

doi: 10.1101/2023.01.19.524842

Figure Lengend Snippet: (A) Western blotting was performed to detect the expression of Axl, Gas6, ZO-1, and occludin in Caco-2 cells infected with K. pneumoniae in the presence of an Axl inhibitor (R428) or an anti-Gas6 antibody. Prior to K. pneumoniae infection, cells were treated for 3 h with Axl inhibitor (R428; 20 nM) or 1 μg of anti-Gas6 antibody. (B and C) Axl inhibitor (R428; 20 nM) or 1 μg of anti-Gas6 antibody were added to Caco-2 cells grown on the insert in in the presence or absence of RAW264.7 macrophages for 3 h prior to K. pneumoniae infection. Next, Caco-2 cells were immunostained with an anti-ZO-1 antibody (B), an anti-occludin antibody (B), and an anti- Klebsiella pneumoniae antibody (C). Scale bar = 50 μm. Immunostaining intensities of ZO-1 (B), occludin (B), and K. pneumoniae (C) were analyzed by ImageJ software. Data are presented as the mean ± SD. ** p < 0.01.

Techniques: Western Blot, Expressing, Infection, Immunostaining, Software

Journal: bioRxiv

Article Title: Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae , to the liver

doi: 10.1101/2023.01.19.524842

Figure Lengend Snippet: (A and B) Sections of cecal mucosa (A) or liver (B) were from mice aged 15 or 57 weeks at 2 days after infection with K. pneumoniae ATCC43816 pmCherry and immunostained with an anti-Gas6 antibody and an anti-Axl antibody. Scale bar = 50 μm. (C) Detection of Axl, Gas6, ZO-1, and occludin by western blotting. Cecum and liver tissues were collected, homogenized, and analyzed using an anti-Axl antibody, an anti-Gas6 antibody, an anti-ZO-1 antibody, or an anti-occludin antibody. Signal intensity was analyzed by ImageJ software. Data are presented as the mean ± SD (n = 5 per group). NS: not significant, * p < 0.05, ** p < 0.01. (D) Linear correlation between Gas6 expression and Axl expression in the cecum or liver of mice aged 15 (red circles) or 57 (blue circles) weeks infected with K. pneumoniae ATCC43816 pmCherry. r > 0.70 and p < 0.05. (E) The population of CD11b+F4/80+ macrophages in the intestinal mucosa of young (14-week-old) and old (56-week-old) mice was examined by staining with anti-F4/80 and anti-CD11b antibodies. The percentage of F4/80-positive/CX3CR1-negative cells in 14-week-old and 56-week-old mice was 29.5% and 8.87%, respectively. Data are representative of four mice.

Techniques: Infection, Western Blot, Software, Expressing, Staining

Journal: bioRxiv

Article Title: Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae , to the liver

doi: 10.1101/2023.01.19.524842

Figure Lengend Snippet: (A) Administration of Gas6 recombinant protein to Caco-2 cells to analyze the expression of Axl, ZO-1, and occludin. Addition of Gas6 recombinant protein to the apical surface (left scheme) or the basolateral side (right scheme) of Caco-2 cells. (B) Western blot analysis to detect Axl, Gas6, ZO-1, and occludin in Caco-2 cells treated with human Gas6 recombinant protein from the apical or basolateral sides. (C) Western blot analysis was performed to detect Axl, Gas6, ZO-1, and occludin. Gas6 recombinant protein (1 μg) was added to the apical side of Caco-2 cells grown in a Transwell co-culture system for 3 h prior to K. pneumoniae infection. (D and E) Gas6 recombinant protein (1 μg) was added to Caco-2 cells grown on the Transwell insert for 3 h prior to K. pneumoniae infection. Next, Caco-2 cells were immunostained with an anti-ZO-1 antibody (C), an anti-occludin antibody (C), an anti- Klebsiella pneumoniae antibody (D), and an anti-E-cadherin antibody. The signal intensities of ZO-1, occluding, E-cadherin, and K. pneumoniae were analyzed by ImageJ software. Data are presented as the mean ± SD. NS: not significant, ** p < 0.01.

Techniques: Recombinant, Expressing, Western Blot, Co-Culture Assay, Infection, Software

Journal: bioRxiv

Article Title: Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae , to the liver

doi: 10.1101/2023.01.19.524842

Figure Lengend Snippet: (A) Treatment scheme used to analyze the effect of Gas6 recombinant protein on the susceptibility of 57-week-old mice to infection by K. pneumoniae . Antibiotics were administered 4 weeks before administration of Gas6 recombinant protein. Gas6 recombinant protein (125 μg protein/kg) was administered intraperitoneally to 57-week-old mice three times every 24 h prior to bacterial infection. (B) Effect of Gas6 recombinant protein on survival of 57-week-old mice infected with K. pneumoniae ATCC43816 pmCherry. Each mouse was orally inoculated with K. pneumoniae ATCC43816 pmCherry (5 × 10 7 bacteria). p -values were determined using the log-rank test (n = 6 per group). (C) Bacterial counts in cecum and liver tissues were determined 2 days post-infection. Cecum and liver tissues were homogenized in PBS. The homogenates were plated on LB agar containing 400 μg/mL ampicillin and the number of CFU was counted. Data are presented as the mean ± SD. (n = 6 per group). * p < 0.05, ** p < 0.01. (D) Detection of Axl, Gas6, ZO-1, and occludin by western blotting. Cecum tissues were collected, homogenized, and analyzed by western blotting with anti-Axl, anti-Gas6, anti-ZO-1, or anti-occludin antibodies. Signal intensity was analyzed by ImageJ software. Data are presented as the mean ± SD (n = 5 per group). NS: not significant, * p < 0.05, ** p < 0.01.

Techniques: Recombinant, Infection, Western Blot, Software

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gas6 axl signal modulators (R&D Systems)

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